Phyto-chemical analysis and antimicrobial evaluation of aloe vera gel against some human and plant pathogens
Keywords:
Aloe vera gel, phytoconstituents, antimicrobial activity, human and plant pathogensAbstract
Aloe Vent leaf gel is widely used as a traditional folk medicine for the treatment of different infectious diseases. Many of the health benefits associated with Aloe vera have been attributed to the polysaccharides present in the gel of leaves. The study is aimed to investigate the phytoconstituents and antimicrobial activity of aqueous, ethanol and acetone extracts of the Aloe vera gel against some human and plant pathogens by the diffusion method. The extracts were screened for the presence of endotoxm contamination by Limukisamebocyte lysate test. The study showed that among three extract ethanol and acetone extracts recorded significant antimicrobial activity against all test pathogens. Antibacterial and antifungal activity of the acetone extract was found to be quite impressive as compared to ethanol and aqueous extracts. Ethanol extract showed high inhibition activity against Bacillus Subtilis (15.10 ± 0.01), Micrococcus kristinae (5.22±0.02) compared to acetone extract and low inhibition activity against Candida albicans (9.96 ± 0.2). Microsporumcanis (7.27 ± 0.02) surprisingly aqueous extract failed to show significant activity against all Bacillus Subtilis (7.96 ± 0.01). Bacillus Cercus (6.89±0.03), Staphylococcus aureus (7.89 ± 0.02), Staphylococcus pyogenes (8.0 ± 0.01), Proteus mirabilis (7.23±0.01), Aspergillums fumigates (6.93±0.13). Aspergillums niger (7.16 ± 0.2). Aspergillums glaceans (7.25 ± 0.02), Tricophytommentagrophyte (6.69±0.20), it showed very low inhibition activity indicating that the active principle responsible for antimicrobial activity is more soluble in organic solvents/The results suggested that Aloe vera gel extract with acetone can be used as antimicrobial agent against human and plant pathogens for medication, cosmetic and food purposes.
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