https://dzarc.com/phytology/issue/feedInternational Journal of Phytology Research2026-05-19T12:41:47+00:00Dzarc Publications (Mamta Kamlashankar)dzarc.phyto@gmail.comOpen Journal Systems<p><strong>International Journal of Phytology Research (IJPR)</strong> is an international, peer-reviewed, refereed and open-access journal, publishing high-quality research in <strong>plant and agricultural sciences</strong>. It offers a global platform for scholars and professionals to share innovative findings across Agronomy, Botany, Horticulture, Forestry, Plant Biotechnology, Phytochemistry, Pharmacognosy, and related fields.</p>https://dzarc.com/phytology/article/view/940Biological suppression of early blight disease (Alternaria solani Sorauer) in potato (Solanum tuberosum L.) using Bacillus subtilis (Ehrenberg) Cohn, Trichoderma harzianum Rifai and Nano Silica2026-05-19T12:41:47+00:00Lilis Irmawatiea@gmail.comRosyad Nurdina@gmail.comDelbiyan Yusupa@gmail.com<p><strong>Background: </strong>Early blight disease, incited by the soilborne necrotrophic fungus <em>Alternaria solani</em> Sorauer, constitutes a major biotic constraint on potato (<em>Solanum tuberosum</em> L.) cultivation, with potential yield losses exceeding 86%. Repeated application of broad-spectrum synthetic fungicides has raised growing concerns regarding environmental contamination, pathogen resistance development, and agrochemical residues in food commodities.</p> <p><strong>Methods: </strong>A screenhouse experiment was carried out from January to May 2024 at the UPTD Seed Potato Station, Pangalengan, West Java, Indonesia (1,500 m a.s.l.; 18–25 °C). A Randomized Block Design was employed with seven treatments and four replications (28 plots; 12 plants per plot; cv. Granola L.). Treatments comprised: P1 – <em>B. subtilis</em> (10 ml/L; 10⁹ CFU/ml); P2 – <em>B. subtilis</em> + Nano Silica (3,000 ppm); P3 – <em>T. harzianum</em> (20 g/L; 10⁶ spores/ml); P4 – <em>T. harzianum</em> + Nano Silica; P5 – <em>B. subtilis</em> + <em>T. harzianum</em> + Nano Silica; P6 – untreated negative control; P7 – Mancozeb 80 WP (2 g/L). Applications began at 10 weeks after planting (WAP) and were repeated weekly for four consecutive weeks. Disease intensity was assessed using a 0–5 ordinal scale and converted to a disease index percentage. Data were subjected to ANOVA and mean separation by Duncan's Multiple Range Test (DMRT) at the 5% level.</p> <p><strong>Results: </strong>Treatment effects on disease intensity were highly significant at all post-application observation periods (F = 15.82–194.85; p < 0.001; R² = 0.82–0.98). At 13 WAP, the triple combination P5 (5.60%) was statistically equivalent to the Mancozeb standard P7 (5.72%), both being significantly superior to all other treatments, while the negative control P6 reached 23.56%. Tuber yield in P2 (636 g/plot) significantly exceeded P6 (375 g/plot). Plant height was not significantly affected by any treatment.</p> <p><strong>Conclusion: </strong>The combined application of <em>B. subtilis</em>, <em>T. harzianum</em>, and Nano Silica (P5) effectively suppressed <em>A. solani</em> to a level statistically equivalent to the synthetic fungicide standard, representing a viable eco-friendly alternative for integrated early blight management in potato production.</p>2026-04-06T00:00:00+00:00Copyright (c) 2026