In vivo and in vitro effects of Curcuma longa in normal and cancerous cell line
Keywords:
cell lines, curcumin extract, in vitro, in vivoAbstract
This study aims to determine the active components in the Curcuma longa rhizome's ethanolic crude extract and evaluate the ethanolic extract's cytotoxic capabilities against the L20B and AMN-3 murine mammary cancer cell lines, two malignant cell lines.
lengths of time that cell lines are exposed In a microtitration plate, different concentrations ranging from 1 to 800 μg/ml were generated and measured after 24, 48, and 72 hours under strict sterile conditions.
Chemical analysis of the extract revealed that it contained flavonoids, saponins, glycosids, alkaloids, and cumarines; phenols, resins, volatile oils, tannins, terpins, and steroids produced undesirable outcomes. After 48 hours, all doses of the ethanolic extract of Curcuma longa showed an inhibitory effect on cancer cell lines.
Curcuma longa crude extract in ethanolic form which, while alkaloids, glycosids, and cumarines produced unfavorable outcomes, contain flavonoids steroids, tannins, terpins, volatile oils, resins, phenols, and saponins. Strong cytotoxic effects have been observed by the ethanolic crude extract of C. longa against.
References
Strimpakos AS, Sharma R. Curcumin: Preventive and therapeutic properties in laboratory studies and clinical trials. Antioxidants & Redox Signaling. 2008;10:1-35.
Esatbeyoglu T, Huebbe P, Ernst IM, Chin D, Wagner AE, Rimbach G. Curcumin--from molecule to biological function. Angewandte Chemie International Edition English. 2012;51:5308-5332.
Zandi K, Ramedani E, Mohammadi K, Tajbakhsh S, Deilami I, Rastian Z, et al. Evaluation of antiviral activities of curcumin derivatives against HSV-1 in vero cell line. Natural Product Communications. 2010;5:1935-1938.
Chen CY, Yang WL, Kuo SY. Cytotoxic activity and cell cycle analysis of hexahydrocurcumin on SW 480 human colorectal cancer cells. Natural Product Communications. 2011;6:1671-1672.
Kim MK, Park JC, Chong Y. Aromatic hydroxyl group plays a critical role in antibacterial activity of the curcumin analogues. Natural Product Communications. 2012;7:57-58.
Basile V, Ferrari E, Lazzari S, Belluti S, Pignedoli F, Imbriano C. Curcumin derivatives: molecular basis of their anti-cancer activity. Biochemical Pharmacology. 2009;78:1305-1315.
Kunwar A, Barik A, Pandey R, Priyadarsini KI. Transport of liposomal and albumin loaded curcumin to living cells: an absorption and fluorescence spectroscopic study. Biochimica et Biophysica Acta. 2006;1760:1513-1520.
Kumar V, Lewis SA, Mutalik S, Shenoy DB, Venkatesh, Udupa N. Biodegradable microspheres of curcumin for treatment of inflammation. Indian Journal of Physiology and Pharmacology. 2002;46:209-217.
Tiyaboonchai W, Tungpradit W, Plianbangchang P. Formulation and characterization of curcuminoids loaded solid lipid nanoparticles. International Journal of Pharmaceutics. 2007;337:299-306.
Gupta NK, Dixit VK. Bioavailability enhancement of curcumin by complexation with phosphatidyl choline. Journal of Pharmaceutical Sciences. 2011;100:1987-1995.
Alam S, Panda JJ, Chauhan VS. Novel dipeptide nanoparticles for effective curcumin delivery. International Journal of Nanomedicine. 2012;7:4207-4222.
Mukhopadhyay A, Basu N, Ghatak N, Gujral PK. Anti-inflammatory and irritant activities of curcumin analogues in rats. Agents Actions. 1982;12:508-515.
Paradkar PH, Juvekar AS, Barkume MS, Amonkar AJ, Joshi JV, Soman G, et al. In vitro and in vivo evaluation of a standardized Curcuma longa Linn formulation in cervical cancer. Journal of Ayurveda and Integrative Medicine. 2021;12(4):616-622.
Arun T, Rabeeth M. Genotoxic effect of paracetamol containing tablets in cultured human lymphocytes. International Journal of Biomedical Research, IJBR. 2010;1(2):21-30.
Kadhim TA. Apoptotic activity of paracetamol on normal lymphocytes by DNA fragmentation measurement. Euro. J. Exp. Bio. 2014;4(5):1-6.
Araldi RP, De Melo TC, Mendes TB, De Sá Júnior PL, Nozima BHN, Ito ET, et al. Using the comet and micronucleus assays for genotoxicity studies: a review. Biomedicine and Pharmacotherapy. 2015;72:74-82.
Fairbairn Daryl W, Olive Peggy L, OʼNeill Kim L. The comet assay: a comprehensive review. Mutation Research. 1995;339:37-59.
Liao Wenjuan, McNutt Micheal A, Zhu Wei-Guo. The comet assay: a sensitive method for detecting DNA damage in individual cells. Methods. 2009;48:46-53.
Henning SM, Fajardo Lira C, Lee HW, Youssefian AA, Go VLW, Herber D. Catechine content of 18 teas and a green tea extract supplement correlates with the oxidant capacity. J. Nutrition and Cancer. 2003;45:226-235.
Sugden D, Davidson K. "Melatonin, melatonin receptors and melanophores: a moving story". Pigment Cell Res. 2004;17:454-460.
Kim E, Jeong K, Tae-yoonkim, Shin-Jeong L, Hyun-Ok Y, Sanghwa H, et al. Biochemical and Biophysical Research Communication. 2005;335(2):300-308.
Krishnaswamy K, Polasa K. Non-nutrient and cancer prevention. J. ICMR Bull. 2001;31:1-3.
Li YM, Ohmo Y, Minatoguchi S, Fukuda K, Fujiwora H. Xtract from the roots of Linderastrychifolia induces apoptosis in lung cancer cells and prolongs survival of tumor. Wearing mice. Am. J. Clin. Med. 2003;31:857-869.
Gulcin I, Oktay M, Kufrevioglu OI, Aslan A. Determination of antioxident activity of lichen Cetraria islandica (L.) Arch. J. Ethnopharmacol. 2002;79:325-329.
Palozza P, Serini S, Di Nicuolo F, Calviello G. Modulation of apoptosis signaling by carotenoids in cancer cells. J. Arch. Biochim. Biophys. 2004;430:104-109.
Pacifico F, Leonardi A. NF-kappa B in solid tumors. J. BiochemPharmacol. 2006;72:1142-1152.
Martin-Cordero C, Lopez-Lazaro M, Galvez M, Ayuso MJ. Curcumin as a DNA topoisomerase II poison. J. Enzyme Inhib. Med. Chem. 2003;18:505-509.
Choudhuri T, Pal S, Aggarwal ML, Das T, Sa G. Curcumin induces apoptosis in human breast cancer cells through p53- dependent Bax induction. FEBSLett. 2002;512:334-340.
