Cytotoxic and genotoxic effects of melphalan in normal and cancerous cells
Keywords:
cytotoxic, genotoxic, melphalan, amn-3, hela , l20b cell lineAbstract
Chemotherapy toxicity is a major clinical issue for individuals with advanced cancer. Melphalan is in charge of creating covalent between DNA strands. There exists a correlation between the quantity of intrastrand cross-links created and the drug's in vitro cytotoxicity as well as the patient's response to therapy. These illnesses continue to be a significant public health concern even with the ongoing advancements in the strong therapeutic and pharmacology impact of novel therapy options for cancer patients. Determine the effects of melphalan on both malignant and normal cells, we assessed the cytotoxicity of melphalan derivatives, which revealed increased cytotoxicity. We evaluated the test chemicals' cytotoxic potential against both normal L20B cells and cancerous cells. MEL and its derivatives had dose-dependent cytotoxic effects. The concentration required to 50% block the biological process in vitro was found to be the IC50 value. Chemical structure-biological activity analyses may be based on the results of the conducted study, which also made it possible to choose the molecule with the highest biological activity.
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