Determining the inhibitory effect of Nigella sativa extract and comparing it with some antibiotics on gram-positive and gram-negative bacteria
Keywords:
Nigella sativa, extract, antibiotics, gram-positive, gram-negative bacteriaAbstract
The most typical bacterial infection is a urinary tract infection, and if it is not treated, problems may result. In order to examine, identify, and test the microorganisms responsible for urinary tract infections for susceptibility to antibiotics and Nigella sativa plant extract, 100 urine samples were collected as part of the study. According to the findings of the bacterial culture, all isolates grew positively and displayed two types of growth: growth that was positive for gram stain and growth that was negative for gram stain. The results showed that IPM was the most effective antibiotic, as all the isolates were 100% sensitive to it, and the least effective was the CRO antibiotic, as most of the isolates were 100% resistant to it. The results also showed the effectiveness of Nigella sativa extract against the isolates under study and at all concentrations used.
References
Abdullah BH. Prevalence and antimicrobial susceptibility pattern of methicillin-resistance Staphylococcus aureus (MRSA) in AL-Yarmouk teaching hospital – Baghdad. AJPS J., 2013, 13(1).
Ahmed, Osama Ibrahim, Khurshid, Berri Ahmed, Nawab Al-Din, Faten Muhammad. Effect of tannin extract of some plants on the activity of some pathogenic bacteria. College of Agriculture - University of Kirkuk, 2010.
Al-Halim, Saba Muayad Suleiman Muhammad. The inhibitory effect of a number of medicinal plants and some of their active components on some types of salmonella isolated from patients with diarrhea, master's thesis, Faculty, 2001.
Al-Juma, Israa Taha Qassem. Prevalence of Staphylococcus aureus strains resistant to the antibiotic vancomycin and the effect of the CO2 laser in this resistance. Master Thesis, College of Science - University of Mosul, 2012.
Al-Mebairik NF, El-Kersh TA, Al-Sheikh YA, Marie MAM. A review of virulence factors, pathogenesis, and antibiotic resistance in Staphylococcus aureus. Clin. Microbiol. Rev. 2016;27:50-56.
Al-Nasiri, Osama Nijres. Molecular bacteriological and genetic study of Staph bacteria. aureus and studying its resistance to beta-lactam antibiotics and modern quinolones, PhD thesis, University College of Sciences Al-Mustansiriya, 2002.
Al-wazni WS, Al-dwri S, Darweesh MF. The antibacterial∙ & antibiofilm activity of Punica granatum peel aqueous extract against some oral pathogen, Magazin of Al-Kufa University for Biology, 2014, 6(2).
Bonev BB, Brown NM. (Eds.). Bacterial resistance to antibiotics: from molecules to man. John Wiley & Sons, 2019.
Braga LC, Shupp JW, Cumming C, Jett M, Takahashi JA, Carmo LS. Pomegranate extract inhibits Staphylococcus∙ aureus growth and subsequent enterotoxin production.J Ethnopharmacol. 2005;96:335-339.
Chambers HF. The changing epidemiology of Staphylococcus aureus?∙ Emerg Infect Dis. 2001;7(2):178-182.
Cheesbrough M. District Laboratory Practice in Tropical Countries.∙ Second edition update (part 2), Cambridge university press: India, 2012.
Fabricant DS, Farnsworth NR. The value of plants used in tradition medicine for drug discovery. Enviromental Health prespective supplements. 2001;109:69-75.
Hamada, Yasser Hamad. Bacteriological and genetic study of Staphylococcus aureus bacteria isolated from wound infections. Master's thesis, College of Education, Tikrit University, 2008.
Irina S, Vinogrador E, Flahant S, Grigoris. Extraction∙ and purification of fibronectin Staphylococcus aureus, Jour. Infect Immun. 2005;73:3007-3017.
Jawetz E, Brooks GS, Butel JS, Morse SA. Jawetz Melnick∙ and Adeibergs. Medical Microbiology. 23 thed. McGrow Hill, Singafor, 2004.
Li M, Kouzmina E, McCusker M, Rodin D, Boutros PC, Paige CJ, et al. Cytokines and depression in cancer patients and caregivers. Neuropsychiatric Disease and Treatment. 2017;13:2903-2911.
Majid, Guitar Rashid, Al-Shatti, Sabah Malik Habib. The effect of the permeability of some plant extracts on the growth of some microorganisms. College of Agriculture - Basra University, 2002.
Mohammad Hassan J, Bane MY. Evaluation of anti-bacterial activity of punica granatum peels extracts, on growth of gram-positive bacteria isolated from clinical samples, Director General of General Commission for Industrial Research & Development, 2005.
Muhammad Osama Ismail. Prevalence of methicillin-resistant Staphylococcus aureus bacteria among the health staff of some hospitals in Salah al-Din Governorate, diploma thesis - College of Science - University of Tikrit. – Iraq, 2013.
Navarro V, Villarreal ML, Rojas G, Lozoya X. Antimicrobial evaluation of some plant used in Mexican, J. Ethnopharmacology. 1996;53:143-147.
Prescott LM, Harley J, Klein DA. Microbiology. 6thed, McGraw-Hill, USA, 2005.
Rashid Zuhair Najeeb. Medicinal herbs in the civilization of Mesopotamia, Dar Al-Kutub for Printing and Publishing, University of Mosul, Iraq, 2008, 8-10.
Soltani S, Emamie AD, Dastranj M, Farahani A, Davoodabadi A, Mohajeri P. Role of Toxins of Uropathogenic Escherichia coli in Development of Urinary Tract Infection. JPRI. 2018;21(1):1-11.
Souman L. The rapeutic uses of herbs and medicinal plants in traditional. Arab medicine J. The Arab Board of medical specialization. 2001;3(2):53-56.
Stari Francesik, Jerassik Vaclav. Medicinal herbs Translated by: Saad Al-Din Shrooq Muhammad Kazem, first edition, House of General Cultural Affairs, Ministry of Culture and Information, Baghdad, 1986.
Swamy MV, Faraz MAA, Mendem S, Shubham P, Vinyas M. Urinary Tract Infections: A Comprehensive Review. Int. J. Curr. Microbiol. App. Sci. 2020;9(7):773-786.
Terlizzi ME, Gribaudo G, Maffei ME. Uro Pathogenic Escherichia∙ coli (UPEC) Infections: Virulence Factors, Bladder Responses, Antibiotic, and Non-Antibiotic Antimicrobial Strategies. Front Microbiol. 2017; 8(1566):1-23.
Vahabi S, Najafi E, Alizadeh S. Invitro antimicrobial effects of some∙ Herbed essences against oral pathogens. J of Med Plants Res. 2011;5(19):4870-4878.
Yamada H, Chiba A, Matsumuva K, Inazu T, Shimizu T, Kusunoki S, et al. Structures of Sialylated OLinked Oligosaccharides of Bovine Peripheral Nerve - Dystroglycan J. Biol. α Chem. 1997;272:2156-2162.
Zaidan, Israa Ali. Bacteriological and genetic study of Staphylococcus aureus resistant to the antibiotic vancomycin, Master Thesis, College of Science, University of Baghdad, 2007.
