A comparative analysis of photosynthetic pigment elution efficiency by different organic solvents of varying concentrations on leaves of Tecoma stans (L.) Kunth


  • Girish Kumar E Post Graduate, Department of Botany, Mahatma Gandhi Govt Arts College, Chalakkara, Mahe, U.T. of Puducherry, India
  • Vijina K. K. Department of Botany, Mahatma Gandhi Govt Arts College, Chalakkara, Mahe, U.T. of Puducherry, India
  • Sasikala K Department of Botany, Mahatma Gandhi Govt Arts College, Chalakkara, Mahe, U.T. of Puducherry, India


eluent, Tecoma stans, chlorophyll-a, chlorophyll-b, carotenoids, butyl alcohol


Quantitative analysis of photosynthetic pigments using different organic solvents and different concentrations of the same was conducted on leaves of Tecoma stans. The organic solvents used were Acetone, Methanol, Chloroform, Ethanol and Butyl alcohol of 40 and 80 percentage concentrations. From the study it was understood that Methanol at 40% was the best eluent of chlorophyll-a, chlorophyll-b, total chlorophylls and carotenoids. Butyl alcohol at 80% was the weakest eluent of chlorophyll-a, chlorophyll-b and total chlorophylls; where as Methanol at 80% was giving minimum elution of carotenoids.


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Lichtenthaler HK. Chlorophylls and carotenoids: Pigments of Photosynthetic biomembranes, Method. Enzymol. 1987;148:350.

Arar EJ. In-vitro determination of Chlorophylls a, b, c1+c2 and Pheopigments in marine and freshwater algae by Spectrophotometry, U.S. EPA, Rev. 1997;1(2):446.0-1.

Barlow RG, Mantoura RFC, Gough MA, Fileman TW. Pigment signatures of the phytoplankton composition in the northeastern Atlantic during the 1990 spring bloom. Deep-Sea Res. 1993;2(40):459.

Dos Santos ACA, Calijuri MC, Moraes EM, Adorno MAT, Falco PB, Carvalho DP, et al. Comparison of three Methods for Chlorophyll determination: Spectrophotometry and Fluorimetry in Sample containing Pigment mixtures and spectrophometry in samples with separate pigments through High Performance Liquid Chromatography. Acta Limnol. Bras. 2003;15(3):7.

Flander PV, Senka T, Alenka M, Marijan A. Effects of mucilage event on the distrubition of summer phytoplankton as reflected by phytoplankton pigments. Periodicum Biologorum. 2000;102(2):169.

Hallegraeff GM. Seasonal study of phytoplankton pigments and species at a coastal station off Sidney: Importance of diatoms and the nanoplankton. Mar. Biol. 1981;61(2-3):107.

Dere S, Gunes T, Sivaci S. Spectrophotometric Determination of Chlorophyll - A, B and total Carotenoid Contents of some Algae Species using different solvents. Turk. J. of Botany. 1998;22:13.

Shakih SD, Dongare M. Analysis of photosynthesis pigments in Adiantum lunulatum burm. At different localities of Sindhudurg District (Maharastra), Indian Fern J. 2008;25:83.

Porra RJ. Recent advances and re-assessments in Chlorophyll extraction and assay procedures for terrestrial, aquatic, and marine organisms, including recalcitrant algae. In: Scheer H (ed) Chlorophylls, 1991, 31.

Vicas SI, Laslo V, Pantea S, Bandict GE. Chlorophyll and carotenoids Pigment from Mistletoe (Viscum album) leaves using different solvents, Fascicula Biol. 2010;2:213.

Richardson AD, Duigan SP, Berlyn GP. An evaluation of non-invasive methods to estimate foliar chlorophyll content. New Phytologist. 2002;153:185.

Goodwin TW. Studies in Carotenogenesis.24. The changes in Carotenoid and Chlorophyll pigments in the leaves of deciduous trees during autumn necrosis. Biochemical Journal. 1958;68(3):503.

Vechetel BW, Ruppel HG. Lipid Bodies in Eremosphaera viridis DeBary (Chlorophyceae). Plant and Cell Phys. 1992;31:41.

Verma V. Textbook of Plant Physiology. First edit., Published by Ane Books, India, 2008.

Srivastava HS. Plant Physiology, Biochemistry and Biotechnology. First Edition, Rastogi publications, India, 2010.

Nayek S, Choudhury IH, Jaishee N, Roy S. Spectrophotometric Analysis of Chlorophylls and Carotenoids from Commonly Grown Fern Species by Using Various Extracting Solvents. Research Journal of Chemical Sciences. 2014;4(9):63.

Lichtenthaler HK, Wellburn AR. Determination of Total Carotenoids and Chlorophylls A and B of Leaf in Different Solvents, Biol. Soc. Trans. 1983;11:591.

Wright SW, Jeffrey SW, Mantoura RFC. Evaluation of methods and solvents for Pigment analysis. In: Phytoplankton pigments in oceanography: guidelines to modern Methods, UNESCO Publ., Paris, 1997, 261.

Rowan KS. Photosynthetic pigments of algae. Cambridge University Press, Cambridge, 1989.

Porra RJ, Thompson WA, Kreidemann PE. Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectrometry. Biochim. Biophys. Acta. 1989;975:384.

Scheer H. Chlorophylls CRC Press Boca Raton, Ann Arbor, Boston, Landon, 1991, 31.

Ritchie RJ. Consistent sets of spectrophotometric chlorophyll equations for acetone, methanol and ethanol solvents, Photosynth Res.2006;89:27.

Manuela AC, Gheorghe C, Gabriela N. Studies concerning the extraction of Chlorophyll and total Carotenoids from vegetables. Romanian Biotechnological letters. 2014;17(5):7702.

Ikbal MB, Sargul AK, Aroul MA. Comparative the different solvents and Spad Chlorophyll Meter for determination Some Photosynthesis Pigments of Bean and Cowpea Plants. Journal of University of Duhok. 2016;19(1):381.

Morna A. Chlorophyll and Carotenoid content in lettuce (Lactuca sativa L.) and Nettle leaves (Urtica dioica L.). Analele Universitaţii din Oradea, Fascicula: Ecotoxicologie, Zootehnie şi Tehnologii de Industrie Alimntara. 2015;12(1):243.

Sukran D, Nurhayat D, Didem K, Gamze Y, Egemen D. The determination of total protein, total soluble carbohydrate and pigment contents of some macro algae collected from Gemlik-Karacaali (Bursa) and Erdek-Ormanli (Balikesir) in the sea of Marmara. Turkey Oceanologia. 2003;45(3):453.




How to Cite

G. Kumar E, V. K K, and S. K, “A comparative analysis of photosynthetic pigment elution efficiency by different organic solvents of varying concentrations on leaves of Tecoma stans (L.) Kunth”, Int. J. Phytol. Res., vol. 3, no. 1, pp. 14–18, Feb. 2023.