Evaluation of the effect of patient related factors on bacterial periodontitis in Salah al-Din city in Iraq
Keywords:
gingivitis, swab, ampicillin, Streptococcus pyogenes, urbanAbstract
One of the most common bacterial infections in people is periodontal disease. its primary reasons for tooth loss. The primary cause of these oral ulcers is the colonization of harmful microorganisms on the surfaces of the mouth. These microorganisms differ based on the lesion under investigation, the length of their development, and the host.
Aim of the study
The study aimed to evaluate the bacterial causes of periodontitis and determined their antibiotic resistance and explain the relation between the bacterial spp. isolate with different patient’s variables.
Methods
90 specimens were collected from patients with gingivitis resulting from orthodontic and placement from medical clinics and health centers in Balad district in Salah al-Din Governorate after they were diagnosed in bed with gingivitis by specialist doctors. Swabs of the inflamed gums were taken and then cultured on appropriate culture media and appropriate diagnostic tests were performed, the disc diffusion method were used for antibiotics susceptibility test for all isolated bacteria.
Results
The study showed that periodontitis was most prevalent in teenagers (65%) and in female (63.3%) with mixed diet (61.6%) and urban residence (74.7%). Streptococcus pyogenes was most common isolated bacteria from patients (61.9%) also other bacteria were obtained. Strep. pyogenes was sensitive to Ampicillin, Augmentin and Ceftazidime while resist to metronidazole and tetracycline.
Conclusion
The periodontal status is influenced by numerous patient-related local variables. The most resistant bacteria in the current investigation, Streptococcus pyogenes, demonstrated considerable antibiotic sensitivity.
References
Cawson RA. Infective endocarditis as a complication of dental treatment Br. Dent. J. 1981;151:409-414.
Scannapieco FA, Papandonatos GD, Dunford RG. Associations between oral conditions and respiratory disease in a national sample survey population Ann. Periodontol. 1998;3:251-256.
Thoden SK, Van Velzen, Abraham-Inpijn L, Moorer WR. Plaque and systemic disease: a reappraisal of the focal infection concept. J. Clin. Periodontol. 1984;11:209-220.
Destefano F, Anda RF, Kahn S, Williamson DF, Russell CM. Dental disease and risk of coronary heart disease and mortality. Br. Med. J. 1993;306:688-691.
Moore WEC, Moore LV. The bacteria of periodontal diseases. Periodontology. 2000;5(1994):66-77.
Finegold SM, Strong CA, Mc Teague M, Marina M. The importance of black-pigmented gram-negative anaerobes in human infections FEMS Immunol. Med. Microbiol. 1993;6:77-82.
Darveau RP, Tanner A, Page RC. The microbial challenge in periodontitis. Periodontology. 2000;14(1997):12-32.
Haffajee AD, Socransky SS. Microbial etiological agents of destructive periodontal diseases. Periodontology. 2000;5(1994):78-111.
Grenier D, Mayrand D. Adult periodontitis: an ecological perspective of mixed infections Trends Microbiol. 1995;3:148-148.
Forbes BA, Sahm DF, Weissfeld AS, Brawn IA. Bailey and Bailey and Scotts, Diagnostic Microbiology. 12th ed. Mosby. USA. Inc St. Louis, 2007.
Abushaheen MA, Fatani AJ, Alosaimi M, Mansy W, George M, Acharya S, et al. Antimicrobial resistance, mechanisms and its clinical significance. Disease-a-Month. 2020;66(6):10097.
CLSI. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing. 31th Informational Supplement., 2017, 32(3).
Alwan AH, Alanbari BF, Alghazali MW, Hussain AA, Al_Bazaz FAM. Evaluation of the Effect of Patient Related Factors on Periodontal Condition in a Sample of Iraqi Population: A Retrospective Study, 2023.
Jasim HH, Al-Jebouri MM. The relationship between periodontal disease and predisposing factors. Tikrit Journal for Dental Sciences. 2016;4(1):68-80.
Casarin M, Da Silveira TM, Bezerra B, Pirih FQ, Pola NM. Association between different dietary patterns and eating disorders and periodontal diseases. Frontiers in Oral Health. 2023;4:1152031.
Salazar CR, Laniado N, Mossavar-Rahmani Y, Borrell LN, Qi Q, Sotres-Alvarez D, et al. Better Diet Quality Is Associated with Lower Odds of Severe Periodontitis in US Hispanics/Latinos. J. Clin. Periodontol. 2018;45:780-790. Doi: 10.1111/jcpe.12926.
Hussein F, Sattar MA. Screening of periodontal disease related Bacteria in Iraqi patients and their relationship with salivary TLR2 and IL-6 level. Iraqi Journal of Science, 2023, 3340-3348.
Saleh NF, Abdel-Rahman AM. Evaluation of some antibiotics’ resistance of different oral bacteria types isolated from patients with gingivitis and periodontitis. J. Thi-Qar Sci. 2017;6(3):116.
Koren O, et al. Human oral. gut. and plaque microbiota in patients with atherosclerosis, 2011, 108.
Kryvtsova MV, Kostenko YY. Dominant microbial associations of oral cavity at periodontitis and features of their sensitivity to antibacterial drugs. 2020;14(1):51-62.
Al-Farhan SR, AL-Abdullah AA, Al-Moussawi AA. “Isolation and Diagnosis of Anaerobic bacteria of Periodontitis by Molecular Methods in Diabetic and Non-Diabetic Patients in Basra Province/Iraq”. Sci. J. Med. Res. 2019;3(10):53-63.
Abdel-Aziz SM, Aeron A. Bacterial biofilm: dispersal and inhibition strategies. Scholarena Journal of Biotechnology. 2014;1(1):105.
Peacock SJ, Paterson GK. Mechanisms of methicillin resistance in Staphylococcus aureus. Annual Review of Biochemistry. 2015;84:577-601.
Dörr T. Understanding tolerance to cell wall–active antibiotics. Annals of the New York Academy of Sciences. 2021;1496(1):35-58.
Uribe-García A, Paniagua-Contreras GL, Monroy-Pérez E, Bustos-Martínez J, Hamdan-Partida A, Garzón J, et al. Frequency and expression of genes involved in adhesion and biofilm formation in Staphylococcus aureus strains isolated from periodontal lesions. Journal of Microbiology, Immunology and Infection. 2021;54(2):267-275.
Kryvtsova MV, Király J, Koščová J, Kostenko YY, Bubnov RV, Spivak MY. Determination of biofilm formation and associated gene detection in Staphylococcus genus isolated from the oral cavity under inflammatory periodontal diseases. Stud. 2020;14(3):49-64.
Conceição N, Rodrigues WF, De Oliveira KLP, Da Silva LEP, De Souza LRC, Da de Cunha Hueb Barata Oliveira C, et al. Beta-lactams susceptibility testing of penicillin-resistant, ampicillin-susceptible Enterococcus faecalis isolates: a comparative assessment of Etest and disk diffusion methods against broth dilution. Annals of Clinical Microbiology and Antimicrobials. 2020;19:1-5.
Farida Y, Puspita K, Yusvida Z. Empirical Antibiotics Study on Pneumonia in Intensive Care Unit. Geriatrics. 2021;12(13):32-57.
Mijović G, Čizmović L, Vuković MN, Stamatović S, Lopičić M. Antibiotic consumption in hospitals and resistance rate of Klebsiella pneumoniae and Escherichia coli in Montenegro. Acta Clinica Croatica. 2020;59(3):469.
Kityamuwesi R, Muwaz L, Kasangaki A, Kajumbula H, Rwenyonyi CM. Characteristics of pyogenic odontogenic infection in patients attending Mulago Hospital, Uganda: a cross-sectional study. BMC microbiology. 2015;15:1-10.
Haider A, Ikram M, Shahzadi I, Asif Raza M. Antibiotic Drug Resistance. In Polymeric Nanoparticles for Bovine Mastitis Treatment, 2023, p81-110.