Anti-inflammatory gel insulin leaf extract (Tithonia diversifolia) against lymphocyte cell count of periodontitis wistar rats (Rattus norvegicus)
Keywords:inflammatory, insulin leaf, lymphocytes, periodontitis
Background: Periodontitis is an inflammatory disease involving dental support tissue caused by pathogenic microorganisms. Periodontitis can result in damage to the periodontal ligaments and alveolar bones, and the formation of periodontal pockets. Periodontitis can cause inflammation; inflammation is one of the first responses of the body's immune system to infection. Inflammation is triggered by the release of chemical mediators from damaged tissues and cell migration. The inflammatory phase is characterized by chronic inflammatory cells namely macrophages, lymphocytes, and plasma cells. Insulin leaves contain flavonoids that can be used as an anti-inflammatory. This study looked at the anti-inflammatory effect of insulin leaf extract gel against a decrease in the number of lymphocyte cells in periodontitis conditions.
Purpose: To know the effective dose of anti-inflammatory gel insulin leaf extract.
Materials and Methods: Periodontitis Wistar rats were given treatment with insulin gel leaf extract (Tithonia diversifolia) with a concentration of 25%, 50%, and 75%, then the lymphocyte cells were counted using a light microscope.
Results: There were significant differences in lymphocyte cell counts in the control group and three treatment groups with concentrations of 25%, 50%, and 75%.
Conclusions: There were differences in the number of lymphocytes in Wistar rats with periodontitis after administration of insulin leaf extract gel with those that were not given insulin leaf extract gel and concentrations of 75% were the most effective dose to reduce the number of lymphocytes in periodontitis.
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