Detection of HSP27 and TLR4 in surgical patients
Keywords:
post-operative infection, HSP27, TLR4Abstract
Background: Postoperative infection is a common healthcare-associated problem, and unfortunately, a serious complication in all surgery patients. Toll-like receptors (TLRs) are crucial in activating non-specific immunity mechanisms and integrating elements of the immune system, due to interactions between specific and non-specific responses.
Aim of the study: The purpose of this study to evaluated the level of Heat shock protein (HSP27) and Toll-like receptors (TLR4) after different type of surgery and compare with control.
Materials and methods: This study was conducted in the city of Balad, and 60 samples were collected from patients in Balad General Hospital and Primary health care centers; during August 15, 2023, to November 1, 2023 of both sexes and different age group (15 males and 45 females-aged (5 to 50) were split into two groups, patients (60 sample) and healthy groups (30 sample). The immunological parameter tested by ELISA.
Results: The study showed significant increase TLR4 in patient after surgery in compared with control group that were (222.3±88.0 and 107.2±47.3) respectively, at p-value (0.0004). In addition significant increase HSP-27 levels in Patient after surgery in compared with Control group that were (18.00±7.11and 12.60±5.83) respectively. Also this study showed no differences in the level of HSP27 and TLR4 according to gender.
Conclusion: The present study concluded increase TLR4 and HSP27 levels in patient after surgery as compared with healthy group. In addition, no significant differences in the mean of TLR4 and HSP27 between male and female.
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