Partial purification of ALP from serum of obesity patients in Tikrit city
Keywords:
Partial purification, ALPAbstract
This study was done for partial purification of Alkaline phosphatase from the serum of obesity patients using the ion exchange chromatography technique, using DEAE- cellulose, and the Gel filtration technique, using Sephadex G 100. The results showed three peaks for purified ALP after elution by tris - HCl buffer solution. The first peak appeared between (3-10) tubes, the second active peak appeared between (13-15) tubes, and the third active peak appeared between (21-23) tubes. In the ion exchange technique, the ALP activity was still higher than normal level, but there was a decrease as compared with ALP activity in crud enzyme; the decrease was from (120 IU/l) after the ammonium sulphate concentration step to (85, 63, 71 IU/l) after ion exchange chromatography. The results of gel filtration indicate that three protein peaks also appear due to the enzyme alkaline phosphatase and the enzyme ALP. Upon tracking the activity of the ALP enzyme, it is found that the peaks give the high specific activity of the enzyme, reaching (77, 59, and 37) enzyme unit/mg, respectively.
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