Isolation, identification and antimicrobial susceptibility profile of Pseudomonas species isolated from pig (Sus scrofa) feces in owo metropolis
Keywords:
pseudomonas species, pig feaces, owo, resistance, organismsAbstract
Pseudomonas species are common pathogenic Gram negative bacteria frequently found in environmental samples. Pseudomonas species are responsible for different healthcare-associated infections and are inherently resistant to many commonly used antibiotics. This study was aimed at isolating; identifying and determining the antimicrobial susceptibility pattern of Pseudomonas species isolated from pig feaces in Owo metropolis. Freshly passed feacal samples were aseptically collected from apparently healthy pigs into appropriately labelled sterile capped universal bottles with sterile spatula from a private owned pig farms in Owo. Isolation of Pseudomonas species was done using Centrimide Agar, however their morphological and cultural characteristics on Nutrient Agar, MacConkey Agar, and Eosin Methylene Blue Agar were also observed; and the isolates were subsequently conventionally characterized. Antimicrobial susceptibility of the isolates was done using Kirby Bauer disc diffusion method. The isolates showed different morphological and cultural characteristics on the different types of media used in their study. The isolates showed varying level of resistance to the antibiotics tested with the lowest resistance (16.7%) to the carbapenem (imipenem) and the highest resistance (83.3%) to both the beta-lactam combination (augmentin) and one of the cephem (cefuroxime) respectively This necessitates the implementation of mitigating strategies to limit the transfer of antibiotic-resistant Pseudomonas species from animals to humans.
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