Fusarium soil isolate shows increased IAA production under variable culture parameters
DOI:
https://doi.org/10.64171/IJPR.2026.6.1.18-23Keywords:
Auxin, Culture medium, Fusarium, Carbon, Nitrogen, pH, TemperatureAbstract
The current study reports the effect of various culture parameters on the indole acetic acid (IAA) by non-pathogenic Fusarium sp. isolated from an agricultural field of Murshidabad district in West Bengal, India. Auxin is one of the principal plant growths promoting substances that influences the overall growth developmental processes in angiosperms. The Fusarium sp. was isolated by soil dilution plate technique on selective pentachloronitrobenzene (PCNB) medium. The isolate was identified and subsequently characterized by the routine morphological studies. Various physiological parameters viz., growth media, carbon and nitrogen sources, incubation temperature and pH of the culture medium were taken into account for determining their effect on IAA production and subsequent optimization of the culture conditions. IAA production was found to be highest in CD broth and Asthana and Hawker’s broth equally (380 µg/ml). Among the carbohydrate and nitrogen sources used, maltose (390 µg/ml) and sodium nitrate (390µg/ml) respectively were found to be most conducive for IAA production. Interestingly, in acidic pH-4, IAA production was completely ceased. Maximum IAA production was recorded at pH-7 (400 µg/ml) while optimum temperature for IAA production was found as 270 C (390 µg/ml). Though the isolate was found quite promising in producing IAA in wide ranges of temperature and pH. This IAA producing Fusarium isolate could turn out to be a potential candidate for improving agricultural output.
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