Phytochemical characterization, antioxidant and antimicrobial testing of ethanolic and ISO-propanolic extracts of Annona squamosa seeds
Keywords:
antioxidant activity-Annona squamosa, DPPH assay, phytochemical analysis, antimicrobial activity- Annona squamosa, agar well diffusion assayAbstract
Annona squamosa, widely known as custard apple or sugar apple, is extensively consumed for its creamy white pulp having a sweet, tropical taste. The aim of this study is to identify the phytochemical constituents, antioxidant and antimicrobial testing of ethanolic and isopropanolic extracts of Annona squamosa seeds. The DPPH radical scavenging assay was used for antioxidant testing. It showed that the isopropanolic extract has high antioxidant potential (IC50 11.76mg/ml) while ethanolic has appreciable antioxidant potential with IC50 12.69 mg/ml. The IC50 value of control was 10.81 mg/ml. Qualitative analysis by phytochemical screening confirmed the presence of steroids, alkaloids, phenols and terpenoids. Antimicrobial testing of the seed extracts was done using the agar well diffusion assay, against the organisms Klebsiella pneumoniae, Staphylococcus aureus and Staphylococcus epidermidis. The isopropanolic and ethanolic extracts showed notable zones of inhibitions. The study concluded that Annona squamosa seeds are a potential antioxidant and antimicrobial agent.
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