Feather degradation by actinobacteria: molecular characterization and biotechnological applications
Keywords:
Actinobacteria, Feather degrading soil, 16S rRNA analysisAbstract
This study focused on the isolation and characterization of keratinase-producing actinobacteria from feather dumping soil. Based on the clearance zones formed on the medium, selected colonies were subjected to secondary screening using a modified basal liquid medium supplemented with raw chicken feathers. Out of ten isolates, only three - JJCPDKTA2, JJCPDKTA3, and JJCPDKTA9 - were further examined for their morphological, physiological, and biochemical characteristics. In addition, 16S rRNA sequencing and phylogenetic analysis identified them as Streptomyces variabilis, Streptomyces azureus, and a member of Actinomycetales. These isolates were deposited in the GenBank database and assigned accession numbers: S. variabilis (KR909307), S. azureus (KR909308), and Actinomycetales sp. (KR909309). The findings demonstrate the potential of these actinobacteria in keratinase production, highlighting their efficiency in feather degradation through keratinolytic activity.
References
Allan RN, Lebbe L, Heyrman J, De Vos P, Buchanan CJ, Logan NA. Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov., two novel thermoacidophiles isolated from geothermal soils of northern Victoria Land, Antarctica. Int J Syst Evol Microbiol. 2005;55:1039-50.
Coward-Kelly G, Agbogbo FK, Holtzapple MT. Lime treatment of keratinous materials for the generation of highly digestible animal feed: 2. Animal hair. Bioresour Technol. 2006;97:1344-52.
Esawy MA. Isolation and partial characterization of extracellular keratinase from a novel mesophilic Streptomyces albus AZA. Res J Agric Biol Sci. 2007;3(6):808-17.
Hoq MM, Siddiquee KAZ, Kawasaki H, Seki T. Keratinolytic activity of some newly isolated Bacillus species. J Biol Sci. 2005;5(2):193-200.
Dhanasekaran D, Selvamani S, Panneerselvam A, Thajuddin N. Isolation and characterization of actinomycetes in Vellar Estuary, Annangkoil, Tamil Nadu. Afr J Biotechnol. 2009;8(17):4159-62.
Kathiresan K, Bingham BL. Biology of mangroves and mangrove ecosystems. Adv Mar Biol. 2001;40:81-251.
Kuster E. Simple working key for the classification and identification of named taxa included in the International Streptomyces Project. J Syst Bacteriol. 1972;22:139-48.
Kumar KS, Haritha R, Mohan YSYV, Ramana T. Screening of marine actinobacteria for antimicrobial compounds. Res J Microbiol. 2011;6(4):385-93.
Lee LH, Cheah YK, Sidik SM, Ab Mutalib NS, Tang YL, Lin HP, et al. Molecular characterization of Antarctic actinobacteria and screening for antimicrobial metabolite production. World J Microbiol Biotechnol. 2012;28:2125-37.
Priya E, Nagasathya A, Steffi PF, Thamilmaraiselvi B. Characterization of actinobacteria isolated from salt pan environment of Thondi, Ramanathapuram Dt. Res J Pharm Technol. 2020;13(11):5108-14.
Priya E, Thenmozhi R, Nagasathya A, PraveenKumar D, Thajuddin N, Muralitharan G. Antagonistic potential of Streptomyces flavomacrosporus GACMPT-57 against plant pathogens. J Microbiol Biotechnol Res. 2014;4(1):68-73.
Priya E, Thenmozhi R, Nagasathya A, Thajuddin N, Muralitharan G. Diversity of actinobacteria in mangrove ecosystem of Muthupet, India. Int J Environ Sci. 2014;3(4):13-7.
Wang JJ, Swaisgood HE, Shih JC. Production and characterization of bio-immobilized keratinase in proteolysis and keratinolysis. Enzyme Microb Technol. 2003;32:812-9.
Wang X, Parsons CM. Effect of processing systems on protein quality of feather meal and hog hair meals. Poult Sci. 1997;76:491-6.
Xie F, Chae Y, Yang X, Yang J, Xue Z, Luo Y, et al. Purification and characterization of four keratinases produced by Streptomyces sp. strain in native human foot skin medium. Bioresour Technol. 2010;101:344-50.
