Role of cyanobacteria in germination and growth of paddy seedlings
Keywords:
cyanobacteria, rice, seed germination, phosphate solubilization, indole acetic acid, exopolysaccharideAbstract
Cyanobacteria are first oxygen evolving prokaryotic photosynthetic organisms used as biofertilizer in several crop fields and play a crucial role to increase crop yields. They were isolated and screened for several plant growth promoting activities like indole acetic acid (IAA) production, phosphate solubilization, exopolysaccharide (EPS) production and their impact on seed germination, vigour index and root/shoot ratio (R/S ratio) of paddy plant. Isolated cyanobacteria Nostoc calcicola, N. punctiforme, N. linckia and Anabaena oryzae was identified using standard monographs. Paddy seeds were treated with different cyanofilterats in which N. punctiforme showed highest 97.33% seed germination while N. calcicola showed 90.66% and control (soaked in DDW) showed only 88% seed germination. Maximum vigour index was showed by N. linkia i.e., 988.26. IAA production was found 0.88 µg/ml in case of N. calcicolawhile phosphate solubilization 94.3 µm in diameter was maximum in N. punctiforme followed by 75 µm, 47.9 µm, 30.7µm in N. calcicola, N. linckia and A. Oryzae, respectively. EPS production was highest in A. oryzae i.e., 0.205 µl/ml and minimum in N. linckia which is 0.108 µl/ml. The data of our study showed that inoculation of these cyanobacterial species to paddy seedlings appear a potential candidate to promote seed germination, vigour index and R/S ratio of paddy plant. These results encourage use of A. oryzae, N. calcicola, N. linckia and N. punctiforme as biofertilizer for rice crop to enhancing growth without using harmful chemical fertilizers.
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