The effect of extract gel of pandan wangi leaves on level of CD34 post-gingival incision
Keywords:
incision gingiva, wound healing, CD34, pandan wangi leavesAbstract
An incision is a common procedure applied in dentistry. Incision leads to blood vessel damage in tissue, followed by wound healing process. Wound healing requires angiogenesis process in order to distribute nutrients to the tissues. One of the angiogenesis molecule markers is Cluster of differentiation 34 (CD34). CD34 is an antigen used as an indication of the new blood vessel growth by endothelial cells. Pandan Wangi leaves have antioxidant compounds that stimulates angiogenesis process and therefore accelerate formation of blood vessels. The purposes of this study were to determine the effect of Pandan Wangi leaves extract gel in various concentration (12.5%, 25%, and 50%) on CD34 levels in the wound healing process post-gingival wound incision of wistar rats. The study was experimental laboratory with posttest-only control group design, using 30 male rats Rattus Norvegicus wistar strain that separated into 5 groups, healthy control group (K0), negative control group (K1), treatment groups of 12,5% gel extract concentration (P1), 25% (P2), and 50% (P3). In each group, peripheral serum blood samples were taken on day seven to examine the CD34 levels using ELISA method. The results showed that there were significant differences among the treatment groups (p<0.05). Post-Hoc analysis showed significant differences between treatment group 2 and 3 and negative control group (p<0.05). However, there was no significant differences between treatment group 1 and negative control group (p>0.05). Following this study, it was concluded that an increase of CD34 levels in wound healing post-gingiva incision of Wistar rats were affected by application of pandan wangi leaves extract gel at concentration 25% and 50%.
References
Abdurrahmat AS. Luka, Peradangan, dan Pemulihan. Jurnal Entropi. 2014;9(1):729-738.
Wolf H, Aamont G, Jerkins S. Periodontology. Theme: New York, 2006.
Singer. Cutanoeus Wound Healing. England Journal Medicine. 1999;341(3):738-754.
Broughton G. Wound Healing. Plastic, and Reconstructive Surgery Journal. 2006;117(7S):12S-34S.
Yulianti H, Bethy S. Hubungan Immunoekspresi CD34 Terhadap Gradasi dan Stadium Duke Pada Adenoma Kolorektal. Patologi Anatomi FK UNPAD. 2013;45(4):240-244.
William D. Advances in Wound Care and Healing Technology. Am J Clin Dermato. 2003;1(2):5.
Asahara T. Isolation of Putative Progenitor Cell for Angiogenesis. Science. 1997;275(964):964.
Nielsen J. Novel Function of CD34 Family, Journal of Cell Science. 2008;121(22):3683-3684.
Sunarjo L. Manfaat Xanthone Terhadap Kesembuhan Ulkus Rongga Mulut Dilihat dari Jumlah Sel PMN dan Fibroblast. ODONTO Dental Jurnal. 2015;2(2):15.
Dalimartha S. Tanaman Obat di Lingkungan Sekitar, Puspa Sehat, Jakarta, 2002, h23.
Hariana. Tumbuhan Obat dan Khasiatnya. Penebar Swadaya: Jakarta, 2008, h265-266.
Prameswari O, Widjanarko B. Uji Efek Ekstrak Air Daun Pandan Wangi Terhadap Penurunan Kadar Glukosa Darah dan Histopatologi Tikus Diabetes Mellitus, Jurnal Pangan dan Agroindustri. 2014;2(2):17-25.
Nofikasari I, Afifah R, Chyntia D, Failasofia. Efek Aplikasi Topikal Gel Pandan Wangi Terhadap Penyembuhan Luka Gingiva. Majalah Kedokteran Gigi. 2016;2(2):53-59.
Zatz J, Kushla G Gels. Marcel Dekkers Inc : New York, 1996.
Rowe C, Sheskey PJ, Owe S. C. Handbook of Pharmaceutical Excipients 4th Ed. Pharmaceutical Press: London, 1996.
Purnama H, Sr Iwidodo, Ratnawulan S. Review sistematik: Proses Penyembuhan dan Perawatan Luka. Farmaka. 2015:15(2):251-259.
Indah P, Ani Bethy. Peran Ekstrak Etanol Topikal Daun Mengkudu (Morinda citrifolia L.) Pada Penyembuhan Luka Ditinjau dari Imunnoekspresi CD34 dan Kolagen pada Tikus Galur Wistar. Majalah Kedokteran Bandung. 2013;45(4):226-233.
Dasopang E, Simutuah A. Formulation of Gel Antiseptic Hand and Test Of Antibacterial Activity From Etanol Extract Pandanus Leaf (Pandanus amaryllifolius Roxb). Fakultas Farmasi Universitas Tjut Nyak Dien, Aceh, 2016.
Hasyim N, Faradiba, Baharuddin G. Formulasi Gel Sari Buah Belimbing Wuluh (Averrhoa bilimbi L.). Majalah Farmasi dan Farmakologi. 2011;15(1):5-9.
Clarkson PM, Thomson HS. Antioxidants: What Role Do They Play in Physical Activity and Health. Am Journal Clinic Nutritionist. 2000;729 (Suppl):346-637.
Sozmen B, Kozaz L, Taskiran D, Tuzens. Effect of N- dicycloprorylmethyl Amino 2 Oxazoline (s 3341) on Antioxidant Status and Nitric Oxide in Hypertensive Patients. Current Medical Researh and Opinion. 1998;14(2):89-96.
Winarsi. Antioksidan Alami dan Radikal Bebas, Kanisius, Yogyakarta, 2007.
Acar T, Tcylidiz R, Vahapogxlu H, Karakayali S, Aydin R. Efficansy of Micronized Flavonoid Fraction on Healing in Thermall Injured Rat. Amal of Burns and Fire Disasters. 2002;15(1):1-7.
Izzati U. Efektivitas Penyembuhan Luka Bakar Salep Ekstrak Etanol Daun Senggani Pada Tikus Galur Wistar, Skripsi, Jurusan Farmasi Fakultas Kedokteran Universitas Tanjungpura, Tidak dipublikasikan, 2015.
Diegelmann R, Evans M. Wound Healing: An Overview of Acute Fibrotic and Delayed Healing. Frontiers in Bioscinece. 2004;9:283-289.
